Maize resistance to insect herbivory is enhanced by silencing expression of genes for jasmonate‐isoleucine degradation using sugarcane mosaic virus
نویسندگان
چکیده
Previously, sugarcane mosaic virus (SCMV) was developed as a vector for transient expression of heterologous genes in Zea mays (maize). Here, we show that SCMV can also be applied virus-induced gene silencing (VIGS) endogenous maize genes. Comparison sense and antisense VIGS constructs targeting phytoene desaturase (PDS) showed resulted greater reduction expression. In time course after infection with PDS, lesion mimic 22 (Les22), Iodent japonica 1 (Ij1), efficient observed 2, 3, 4 weeks SCMV. However, at Week 5, Les22 Ij1 no longer significantly reduced compared control plants. The defense signaling molecule jasmonate-isoleucine (JA-Ile) inactivated by 12C-hydroxylation hydrolysis, knockout these leads to herbivore resistance. JA-Ile hydroxylases hydrolases have been investigated Arabidopsis, rice, Nicotiana attenuata. To determine whether the homologs function plant defense, silenced ZmCYP94B1 (predicted hydroxylase) ZmJIH1 hydrolase) SCMV, which elevated two defense-related genes, Maize Proteinase Inhibitor (MPI) Ribosome Inactivating Protein 2 (RIP2). Although increased resistance Spodoptera frugiperda (fall armyworm), Schistocerca americana (American birdwing grasshopper), Rhopalosiphum maidis (corn leaf aphid), there additive effect from both Further work will required more precise functions enzymes regulating defenses.
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ژورنال
عنوان ژورنال: Plant direct
سال: 2022
ISSN: ['2475-4455']
DOI: https://doi.org/10.1002/pld3.407